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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Hum Immunol. 2015 Mar 20;76(5):329–338. doi: 10.1016/j.humimm.2015.03.007

Figure 4. Detection of intracellular cytokine production by non-regulatory T cells.

Figure 4

Umbilical cord blood cells were stimulated using PMA/ionomycin or left un-stimulated, both in the presence of monensin. The flow cytometery gating strategy illustrated in Figure 1 was used to identify the non-T regulatory cells (Dead-Dump- CD3+CD4+CD127+FoxP3-). A gate was then placed to identify the frequency of cytokine producing or activated (CD69+) T cells. Representative data are shown from matched stimulated and un-stimulated patient samples.