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. Author manuscript; available in PMC: 2016 Jan 17.
Published in final edited form as: Nat Commun. 2015 Jul 17;6:7770. doi: 10.1038/ncomms8770

Figure 8. STAT1 regulates chemokines and inflammation in PDGFRβSm22D849V mice.

Figure 8

(a) Expression of total STAT1 and STAT1 p-Y701 in PDGFRβSm22D849V (β-D849V) mutant and Sm22α-Cre (Wt) control VSMCs after transfection with control or STAT1-blocking siRNA was analyzed by Western blotting. (b) Fold change in chemokine mRNA levels in cultured VSMCs from mutant mice was determined by quantitative RT-PCR. Results are shown as fold change in STAT1 siRNA compared to control siRNA, n=4 transfections per siRNA. (c) Expression of total STAT1 and p-Y701 STAT1 in PDGFRβSm22D849V (β-D849V) single mutant and STAT1Sm22CKOPDGFRβSm22D849V (β-D849V,STAT1-cKO) double mutant VSMCs was analyzed by Western blotting. (d) Fold change in chemokine mRNA levels in Wt, STAT1Sm22CKO (STAT1-cKO), PDGFRβSm22D849V (β-D849V) single mutant, and STAT1Sm22CKOPDGFRβSm22D849V (β-D849V,STAT1-cKO) double mutant VSMCs was determined by quantitative RT-PCR. Results are shown as fold change compared to Wt, n=3 different VSMC isolates per genotype. (e) Quantification of CD45+ leukocytes in the whole aorta of single mutant and double mutant mice (all ApoE+/+) as measured by flow cytometry, n=3 mice per genotype. (f) Mature VSMC marker expression in the cross-sectioned thoracic aorta of 8-week old mice was determined by immunofluorescence, with DAPI as a nuclear counterstain. Representative images from three aortas of each genotype are shown. Scale bar, 50μm. All data were assessed using Student’s t-test and are present as mean ± s.e.m. *P<0.01.