FIG. 3.

Early biodistribution of injected iPSCs. (A) Fibrinogen-treated animals showed a rather focused thoracic bioluminescence imaging (BLI) signal, with most of injected iPSCs retained in the heart (B; 1, heart; 2, right lung; 3, left lung). (C) In the medium group, thoracic projection of BLI signal suggests lung biodistribution of injected iPSCs. (D) BLI assessment of organ explants unveiled massive iPSC accumulation in the right and the left lung. (E) Comparison between both groups showed that, in the fibrinogen group, more than 60% of total cells were retained in the heart, whereas in the medium group only 22% of all cells could be found in the heart. (F) Calculation of absolute iPSC amounts also showed significant higher number of intramyocardial iPSCs with lower lung concentrations of injected iPSCs in the fibrinogen group compared with medium controls. Color images available online at www.liebertpub.com/tea