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Immunofluorescent labeling for actin (red), tubulin (green) and DNA (blue) in germinal vesicle (GV)-intact oocytes from in vitro grown follicles. Healthy GV oocytes showed normal chromatin configuration including non-surrounded nucleolus (diffused), partially surrounded nucleolus and surrounded nucleolus. Extensive development of the transzonal projections (TZPs) was also evident in cumulus–oocyte complexes.
