TABLE 2.
Oligonucleotides used for construction of plasmids containing protospacers
| Plasmid | Oligonucleotide sequencea |
|---|---|
| sp4_AAA/TTT | AGCTaaaTTTCAGCATTGTATTGAACTGAACATATAGAGAATCaaa |
| TCGAtttGATTCTCTATATGTTCAGTTCAATACAATGCTGAAAttt | |
| sp4 CCT/TTT | AGCTcctTTTCAGCATTGTATTGAACTGAACATATAGAGAATCaaa |
| TCGAtttGATTCTCTATATGTTCAGTTCAATACAATGCTGAAAagg | |
| sp4 AAA/AGG | AGCTaaaTTTCAGCATTGTATTGAACTGAACATATAGAGAATCagg |
| TCGAcctGATTCTCTATATGTTCAGTTCAATACAATGCTGAAAttt | |
| sp5 AAA/TTT | AGCTaaaAAAGTTTTAAGATTAGCAAACATTTTACCATCTTGTaaa |
| TCGAtttACAAGATGGTAAAATGTTTGCTAATCTTAAAACTTTttt | |
| sp5 CCT/TTT | AGCTcctAAAGTTTTAAGATTAGCAAACATTTTACCATCTTGTaaa |
| TCGAtttACAAGATGGTAAAATGTTTGCTAATCTTAAAACTTTagg | |
| sp5 AAA/AGG | AGCTaaaAAAGTTTTAAGATTAGCAAACATTTTACCATCTTGTagg |
| TCGAcctACAAGATGGTAAAATGTTTGCTAATCTTAAAACTTTttt | |
| sp4scr AAA/TTT | AGCTaaaAGACCTTGCAAGTATATTTGAAACGACTTATTGATAaaa |
| TCGAtttTATCAATAAGTCGTTTCAAATATACTTGCAAGGTCTttt | |
| sp4scr CCT/TTT | AGCTcctAGACCTTGCAAGTATATTTGAAACGACTTATTGATAaaa |
| TCGAtttTATCAATAAGTCGTTTCAAATATACTTGCAAGGTCTagg | |
| sp4scr AAA/AGG | AGCTaaaAGACCTTGCAAGTATATTTGAAACGACTTATTGATAagg |
| TCGAcctTATCAATAAGTCGTTTCAAATATACTTGCAAGGTCTttt | |
| RNA random seq. | GATCCGCGTCTCCTTGCGGGTAGATCGCCGACCGCAGAG |
| GATCCTCTGCGGTCGGCGATCTACCCGCAAGGAGACGCG | |
| RNA C30/sp4 (+/−) | GATCCGATTCTCTATATGTTCAGTTCAATACAATGCTGAAAG |
| GATCCTTTCAGCATTGTATTGAACTGAACATATAGAGAATCG |
a
Lowercase letters indicate PAM sequences.