TABLE 4.

Sequences of protospacer regions of pT-COW derivatives used for conjugation assay^a

Plasmid	5′-end-adjacent sequence	Protospacer sequence (5′–3′)	3′-end-adjacent sequence
sp4_TTT/AAA	TATTCGATTT	GAT TCT CTA TAT GTT CAG TTC AAT ACA ATG CTG AAA	AAAAGCTGGC
sp4 CCT/AAA	TATTCGACCT	GAT TCT CTA TAT GTT CAG TTC AAT ACA ATG CTG AAA	AAAAGCTGGC
sp4 TTT/AGG	TATTCGATTT	GAT TCT CTA TAT GTT CAG TTC AAT ACA ATG CTG AAA	AGGAGCTGGC
sp5 TTT/AAA	TATTCGATTT	ACA AGA TGG TAA AAT GTT TGC TAA TCT TAA AAC TTT	AAAAGCTGGC
sp5 CCT/AAA	TATTCGACCT	ACA AGA TGG TAA AAT GTT TGC TAA TCT TAA AAC TTT	AAAAGCTGGC
sp5 TTT/AGG	TATTCGATTT	ACA AGA TGG TAA AAT GTT TGC TAA TCT TAA AAC TTT	AGGAGCTGGC
sp4scr TTT/AAA	TATTCGATTT	TAT CAA TAA GTC GTT TCA AAT ATA CTT GCA AGG TCT	AAAAGCTGGC
sp4scr CCT/AAA	TATTCGACCT	TAT CAA TAA GTC GTT TCA AAT ATA CTT GCA AGG TCT	AAAAGCTGGC
sp4scr TTT/AGG	TATTCGATTT	TAT CAA TAA GTC GTT TCA AAT ATA CTT GCA AGG TCT	AGGAGCTGGC

^aNucleotides in bold indicate PAM sequences.