TABLE 2.
Primers used in this study
| Primer | 5′–3′ sequencea | Product | Restriction cut site |
|---|---|---|---|
| comF_up_for | GCTAGAGCTCGAACAATATCCTTGC | pUC19Fup | SacI |
| comF_up_rev | GCTAGAATTCCACCCGCAAGGCCGC | pUC19Fup | EcoRI |
| comF_down_for | GCATGAATTCTCGGTCGCGGTTCTG | pUC19Fdown | EcoRI |
| comF_down_rev | CTTATCTAGACGGTCCGAGGACAGA | pUC19Fdown | XbaI |
| comM_up_for | GTACGAGCTCAACCATTGCGAAACG | pUC19Mup | SacI |
| comM_up_rev | TACAGGATCCGAAAGCCACCGTATA | pUC19Mup | BamHI |
| comM_down_for | GCACGGATCCTCGCCGATCTGGACG | pUC19Mdown | BamHI |
| comM_down_rev | GACATCTAGACGGCACCAATGTCGC | pUC19Mdown | XbaI |
| JAB-rec-2-fwd | GTGATCCGGCTCACTCTAGATCAGGTGCG | pUC19EC | XbaI |
| JAB-rec-2-rev | GGCAGGCGTTTTCAAGCTTGTCCAGGG | pUC19EC | HindIII |
| comF_screen_for | GCGGCGTCGTCGTGC | NAb | NA |
| comF_screen_rev | TGCGGGTGGTGTAGATT | NA | NA |
| comM_screen_for | GGATCTAGGCTACTG | NA | NA |
| comM_screen_rev | TCCTGAGCGATGATGC | NA | NA |
| comF_comp_for | TACAGAGCTCCGCCATAGCTGACCC | pComF | SacI |
| comF_comp_rev | CAGTAAGCTTGGTGTAGATTTCGAC | pComF | HindIII |
| comM_comp_for | CGTAGAGCTCCGCTGGCTCGCTTCGG | pComM | SacI |
| comM_comp_rev | CGTAGGATCCCCGAGGTGCTGCTGC | pComM | BamHI |
| comEC_comp_for | GTACGAGCTCCTCCTGGGTGGCGA | pComEC | SacI |
| comEC_comp_rev | TAGTAAGCTTCTCGTCGAGGATCTG | pComEC | HindIII |
| gm_for | GTTATGGAGCAGCAACGA | NA | NA |
| gm_rev | GGAGTAGGTGGCTAC | NA | NA |
| pctrA_F | AGAGAAGCTTCTCTGGTCGCGCATCGG | pCtrAmCherry | HindIII |
| pctrA_R | AGAGGGATCCATCCTGGGTTCTCCGCA | pCtrAmCherry | BamHI |
| Gm_qPCR_for | GGTGGCTCAAGTATGGGCAT | NA | NA |
| Gm_qPCR_rev | CGAAAAGATCAAGAGCAGCCC | NA | NA |
| puhA_qPCR_for | AACGACGACGGCAAGCTCT | NA | NA |
| puhA_qPCR_rev | GCTTCTTCGTTCTCGACCGA | NA | NA |
a
Restriction cut sites (if applicable) are underlined.
b
NA, not applicable.