FIG 4.
DeaD does not influence the Gac/Rsm system to control T3SS gene expression. (A) PrsmY-lacZ and PrsmZ-lacZ reporter activity was measured in wt PA103 and a deaD mutant, normalized to wt PA103 (PrsmY-lacZ = 3,620 Miller units; PrsmZ-lacZ = 544 Miller units). (B) RsmY and RsmZ sRNA quantities were measured in wt PA103 and a deaD mutant using qRT-PCR. RNA levels were normalized to the rimM housekeeping gene and reported as a percentage of wt levels. (C) PtssA1′-′lacZ activity was measured in wt PA103 and rsmA and deaD mutants. All activities were normalized to an rsmA mutant (157 CPRG units).