FIG. 2.

A 2400 dots per inch scan of a prostate section from subject 2 stained with picrosirius red (left) for collagen identification. The histotripsy treatment cavity is evident in the left hemiprostate. The red dots define the region of interest used to calculate the tissue collagen density and tissue area. The empty treatment lesion was excluded from the region of interest. A binary, black and white rendering (right) of the treated hemiprostate is shown with collagen containing pixels displayed as white.