FIG. 2.

Survival of islet allografts treated with iPSC-MSCs and Rapa. Islet graft survival and blood glucose levels in C57BL/6 mice with different treatments are shown, respectively (A, n=6) and (B, n=3). Diabetes was induced in C57BL/6 mice 4 days before transplantation. Four hundred islets isolated from BALB/c mice, with or without 1×10⁶ iPSC-MSCs, were transplanted into recipient mice. The control group received islets alone, the iPSC-MSC group received islets with iPSC-MSCs, the Rapa group received islets following a 0.1 mg/kg/day Rapa treatment from days 0 to 9, and the iPSC-MSCs+Rapa group received islets with iPSC-MSCs and 0.1 mg/kg/day Rapa from days 0 to 9. Graft survival was calculated by the Kaplan–Meier method and compared using a log-rank test. Allograft nephrectomies were performed on mice after 100 days to ensure normal glucose levels had been maintained by the islet allograft.