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Working range for each analyte in the multiplex assay. The dynamic range was calculated by dividing the highest quantified sample by the limit of detection and taking a logarithm (base 10).
| Analyte | Limit of Detection (pg/mL) |
Limit of Detection (fM) |
Highest Quantified Sample (pg/mL) |
Dynamic Range (Orders of Magnitude) |
|---|---|---|---|---|
| MCP-1 | <5.0 | 387 | 18,406 | 3.56 |
| Il-1β | 21.8 | 1211 | 10,000 | 2.66 |
| IL-6 | <3.6 | 151 | 3,310 | 2.96 |
| IL-13 | 15.4 | 963 | 1,380 | 1.95 |
| IL-10 | 103.1 | 5726 | 8,766 | 1.93 |
| TNF-α | <4.8 | 92 | 1,090 | 2.36 |
