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. 2015 Jul 20;5:12241. doi: 10.1038/srep12241

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(A,D) Expression of full-length NBCn2 (n2C and n2G) with HA tagged at the Nt end. (B,E) Expression of Nt-truncated NBCn2 (n2K and ΔN121) with Myc tagged at the Ct end. (C,F) Expression of endogenous Na⁺-K⁺-ATPase probed with anti-α1 to verify equal loading in each lane. Surface proteins were prepared by biotinylation and separated on 10% SDS-PAGE for western blotting.