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. 2015 Jul 20;5:12241. doi: 10.1038/srep12241

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Copyright © 2015, Macmillan Publishers Limited

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(A) Sequence alignment of the Nt of rat NBCe2, mouse NBCn1, and rat NBCn2. (B–D) Cellular localization of the full-length rat HA-NBCe2-c (B), mouse HA-NBCn1-R (C), and rat HA-NBCn2-G (D). (E−G) Cellular localization of the Nt-truncated rat HA-NBCe2-g (E), mouse HA-NBCn1-Q (F), and mutant rat HA-ΔN120-NBCn2 truncated to “LDEI” (G). HA (“YPYDVPDYA”) was tagged at the Nt end of the NCBT proteins. Mouse anti-HA primary antibody in combination of Alexa 488 goat-anti-mouse secondary antibody was used to detect the localization of HA-tagged proteins.