Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 20;5:12241. doi: 10.1038/srep12241

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Full-length and Nt-truncated NBCn2 individually expressed in neuro-2A cells. (B−E) Coexpression of HA-NBCn2-C and NBCn2-K-Myc (B), HA-NBCn2-C and ΔN121-Myc (C), HA-NBCn2-G and NBCn2-K-Myc (D), HA-NBCn2-G and ΔN121-Myc (E) in neuro-2A cells. Full-length NBCn2-C and -G were HA-tagged at the Nt end. Nt-truncated NBCn2-K and mutant “ΔN121” were tagged with Myc (EQKLISEEDL) at the Ct end. Full-length NBCn2 was probed with anti-HA, whereas the Nt-truncated NBCn2 was probed with anti-Myc. Two populations of cells were observed for each case in panels B−E. In a minor population of cells, both full-length and Nt-truncated NBCn2 were primarily colocalized on the plasma membrane. In the other major population of cells, both full-length and Nt-truncated NBCn2 were primarily colocalized in the cytosol.