Table 3.

Comparison of cytotoxic score of various irrigants on hPDLSCs and Mouse fibroblast NIH3T3

	Human PDL cells				Mouse fibroblast
	Mean	SD	95% CI for Mean		Mean	SD	95% CI for Mean
			Lower bound	Upper bound			Lower bound	Upper bound
Control	0.2500	0.50000	0	1.0456	0.0000	0.00000	0.0000	0.0000
Nano-silver	0.1250	0.25000	0	0.5228	0.2500	0.50000	0	1.0456
3% Hypo	2.5000*,**	0.57735	1.5813	3.4187	3.7500*,**	0.50000	2,9544	4,5456

Notes: The mean cytotoxic score of hPDLSCs of the 3% sodium hypochlorite group was significantly higher than control *(P<0.05) and nano-silver group **(P<0.05). The same comparison was performed on mouse fibroblasts NIH 3T3. The mean cytotoxic score of NIH 3T3 of the 3% hypo group was significantly higher than control *(P<0.05) and nano-silver group **(P<0.05). The cytotoxic scores of both cell types in various test solutions were compared by crosstab analysis resulting in a 100% Kappa score.

Abbreviations: hPDLSCs, human periodontal ligament stem cells; PDL, periodontal ligament; hypo, hypochlorite.