Figure 5. C. albicans internalization is followed by fungal outgrowth.
Shown are images of the indicated time points for C. albicans infected mast cells (MOI 0.1). (A) Arrows show an intracellular yeast cell (GFP-expressing C. albicans strain CAI4 pENO1-GFP-CyC1t) replicating inside the mast cell (orange, membrane stain DiI) finally rupturing the plasma membrane as determined by loss of signal. An extracellular C. albicans hyphal tip growing towards a mast cell nudged the host cell and induced collapse of the plasma membrane (*). Complete movies are available as Movie S2 and S3 in the supplemental material. Scale bar, 10 μm.