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. 2015 Jul 20;5:12264. doi: 10.1038/srep12264

Figure 2. EECs differentiate based on previously received inflammatory signals in uninfected recipients.

Figure 2

A. Schematic of EEC sorting strategy. 1,000 CD45.1+ OT-I cells were transferred to uninfected CD45.2+ recipients one day prior to infection with LM-OVA or VSV-OVA. 5 days following infection, CD8+ CD45.1+ KLRG1− CD127− EECs were sorted to ~99% purity. B. EECs were purified, as described in A, from both LM-OVA- and VSV-OVA-infected hosts and transferred to uninfected recipients. CD8+ CD45.1+ transferred EECs were then analyzed for KLRG1 and CD127 expression from splenocytes 3 or 6 days post-transfer. Percentages of SLECs, MPECs, and EECs from each condition are graphed from 3–4 mice. This data is representative of at least 2 experiments.