Figure 4. EECs transferred into infected mice show plasticity by modulating their differentiation according to the infection-induced inflammatory environment of the recipient mouse.

A. EECs were purified, as described in Fig. 2A, following LM-OVA or VSV-OVA infection and transferred into LM-OVA infected recipients that had been infected for 2 days. 6 days following the EEC transfer, CD8+ CD45.1+ transferred EECs in the spleen were analyzed for KLRG1 and CD127. Percentages of SLECs, MPECs, and EECs are graphed for both LM-OVA EECs and VSV-OVA EECs from 3–4 mice. This data is representative of at least 2 experiments. B. Same as A, except recipient mice were infected with VSV-OVA 2 days prior to EEC transfer.