Table 2.
Calibration curve, precession, recovery, and matrix effect of EZ.
| Linear range (nM) |
Concentration (nM) | Intra-day | Inter-day | Recovery | Matrix Effect | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Accuracy (bias, %) |
Precession (CV %) |
Accuracy (bias, %) |
Precession (CV %) |
Mean (%) | SD | Mean (%) | SD | |||
| Plasma | 10,000.00-4.88 | 9.75 | 102.80 | 5.06 | 119.35 | 12.87 | 97.63 | 7.80 | 87.04 | 11.67 |
| 312.5 | 101.20 | 4.97 | 115.89 | 12.50 | 82.71 | 3.37 | 85.50 | 8.59 | ||
| 5,000.00 | 106.65 | 6.20 | 114.73 | 11.05 | 80.50 | 13.95 | 100.70 | 7.63 | ||
| Brain tissue |
10,000.00-4.88 | 9.75 | 91.19 | 12.43 | 89.27 | 4.83 | 98.58 | 12.17 | 90.47 | 13.97 |
| 312.5 | 108.00 | 7.46 | 104.45 | 2.66 | 105.22 | 13.59 | 98.13 | 12.00 | ||
| 5,000.00 | 106.22 | 2.14 | 107.11 | 4.30 | 106.23 | 5.74 | 100.44 | 5.59 | ||
| HBSS buffer |
5,000.00-1.22 | 4.88 | 106.44 | 4.05 | 89.02 | 3.07 | 91.35 | 6.00 | 97.73 | 3.53 |
| 312.5 | 88.88 | 1.89 | 92.13 | 2.20 | 98.11 | 3.23 | 99.93 | 2.12 | ||
| 5,000.00 | 100.5 | 1.84 | 107.34 | 3.77 | 98.83 | 4.96 | 101.97 | 5.57 | ||