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. 2015 Jun 2;15(6):12872–12883. doi: 10.3390/s150612872

Figure 5.

Figure 5

The activation of fluorescence intensity of the R9-QD-miR124a beacons in differentiated P19 cells. The P19 cells were induced to neuronal differentiation for 3 days. (A) qRT-PCR analysis of the expression of miR124a during neuronal differentiation in the P19 cells; (B) Immunocytostaining of the P19 cells by Oct3/4 (stem cell marker) and NeuroD (neuronal marker) antibodies. Red fluorescence indicates Oct3/4 or NeuroD expression, and blue fluorescence indicates DAPI, which stains the nucleus; (C) Undifferentiated and differentiated P19 cells were incubated with the R9-QD-miR124a beacons. Confocal microscopy imaging showed that the fluorescence signal of the R9-QD-miR124a beacons in differentiated P19 cells was significantly activated by endogenous miR124a. Images were merged with DAPI; (D) Fluorescence intensity of the R9-QD-miR124a beacons in P19 cells during neuronal differentiation. Data are displayed as means ± standard error of triplicate samples (* p < 0.05, ** p < 0.005).