Fig. 1.

The NSCLC SK-MES-1 cell line produces exosomes that can be isolated with ultrafiltration of CCM. (a) 5 µg of protein was used for Western blot analysis of isolated exosomes. The presence of canonical exosome proteins, and the absence of Calnexin demonstrates a pure exosome preparation. (b) TRPS analysis demonstrates a size distribution of particles consistent with the size range of exosomes. (c) EM image of exosomes demonstrates cup-shape morphology, size bar=200 nm. CL: cell lysate; E: exosome lysate.