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. 2015 Jul 17;4:10.3402/jev.v4.27031. doi: 10.3402/jev.v4.27031

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© 2015 Richard J. Lobb et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Ultrafiltration recovers more particles compared to ultracentrifugation. (a) Size distribution of particles before density gradient purification. (b) Percentage of particle size ranges from ultracentrifugation and ultrafiltration isolations. (c) Ultrafiltration was shown to significantly increase the recovery of <100 nm particles compared to ultracentrifugation; n=3±SEM, *p<0.05. (d) Western blot analysis of equal volumes from ultracentrifugation and ultrafiltration did not show a large difference in protein markers for exosomes. UC: ultracentrifugation; UF: ultrafiltration.