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. 2015 May 14;66(15):4585–4593. doi: 10.1093/jxb/erv229

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Interaction between OsHrd3 and components of the Hrd1 ubiquitin ligase complex. Immunoprecipitation of OsHrd3-FLAGs. Protein extracts from rice protoplasts expressing OsHrd1-HA, OsOS-9-HA, and OsHrd3-FLAG were subjected to immunoprecipitation using anti-FLAG M2 magnetic beads. The immunoprecipitates were analysed by immunoblot analysis using anti-FLAG–horseradish peroxidase (HRP) and anti-HA–HRP conjugated antibodies. C represents 2% (v/v) of the starting crude lysate used for immunoprecipitation. P represents immunoprecipitated proteins.