Fig. 5.

Aberrant protein body-I (PB-I) in the OsHrd3 KD seeds. Indirect immunohistochemical analysis of rice endosperm (18 DAF) using antibodies against OsTIP3 (a–f), RM1 (g–l), and calnexin (m–r). Signals were detected using a secondary antibody conjugated with Alexa488, which emits green fluorescence (a, d, g, j, m, p). PB-I was stained with rhodamine B (b, e, h, k, n, q). Right panels (c, f, i, l, o, r) are merged images of the left (green fluorescence) and the middle (red fluorescence) panels. Scale bar=5 μm.