Table 4.
Result of linear combination fitting (LCF) of Se K-edge XANES data for leaves of both rice (Oryza sativa L.) and wheat (Triticum aestivum L.) grown for 1 week in nutrient solutions containing 1 μM of either Se(IV) or Se(VI)
The values are the arithmetic means of the two species.
| Exposed to Se(IV) | Exposed to Se(VI) | |||
|---|---|---|---|---|
| Inter-vein | Vein | Inter-vein | Vein | |
| C-Se-C compoundsa (%) | 100 (0) | 100 (0) | 44 (0.3) | 48 (0.4) |
| Uncomplexed Se(IV) (%) | ||||
| Uncomplexed Se(VI) (%) | 56 (0.3) | 52 (0.4) | ||
| R-factor | 0.0011 | 0.0035 | 0.0004 | 0.0008 |
Data are presented for the ‘inter-vein’ and ‘vein’ tissues (see Fig. 6; Supplementary Fig. S7 at JXB online). The values in parentheses show the SE in the calculated values in LCF analysis.
The spatial distribution of two pixel populations (inter-vein and vein) identified by comparing energy intensities (see Supplementary Fig. S2 for an example).
The R-factor is the residual factor generated by the LCF tool in Athena and indicates the goodness of fit, with R-factor=∑i(data–fit)2/∑i(data)2.
a C-Se-C compounds refer to selenomethionine (SeMet) or methylselenocysteine (MeSeCys).