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. 2015 Jul 20;10(7):e0132496. doi: 10.1371/journal.pone.0132496

Fig 3. Effect of dehydroxymethylepoxyquinomicin (DHMEQ) on lipopolysaccharide (LPS)-induced nuclear factor (NF)–kB p65 activation in monocytes from cancer patients with and without DVT.

Fig 3

Nuclear extracts were prepared from monocytes, pretreated and not with DHMEQ (10 μg/mL) for 2 h and after which stimulated with 100 ng/ml LPS for 24 hour, using a Nuclear Extract Kit. The stimulation of monocytes with LPS at 100 ng/mL induce un significant increase of the nuclear NF-kB p65 protein level in all groups or cancer patients DVT+, DVT- and in healthy controls, compared to unstimulated cells, (P<0.0001). NF-kB p65 subunit was significantly higher in cancer patients DVT+ than in those DVT- (P< 0.0001) (t-test). DVT, Deep Vein thrombosis.