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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Trends Immunol. 2015 Jun 17;36(7):410–418. doi: 10.1016/j.it.2015.05.005

Figure 1.

Figure 1

Representative gating protocols for Tfr cells based on CXCR5 and ICOS or on CXCR5 and PD-1 expression. Both strategies pregate on CD4+CD19− FoxP3+cells. “Full minus CXCR5 biotin” indicates staining control in which the CXCR5 biotin is omitted.