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. 2015 Jul 20;10(7):e0132569. doi: 10.1371/journal.pone.0132569

Fig 3. LAAO-Bl activates Bax, caspases 3 and 7 in epithelial kidney cells.

Fig 3

(A) Caspase-3/7 activity was determined in the presence of the fluorogenic Ac-DEVD-afc substrate after treatment with LAAO-Bl (12 h) in the presence or absence of zVAD (10 μM) in MDCK (a) and HK-2 cells (b). (B-C) Western blot analyses were performed to detect the effects of LAAO-Bl on changes in Caspase-3 (a), -7 (b) and Bax (c) expressions from total protein of MDCK (B) and Caspase-3 (a), -7 (b) HK-2 (C) cell extracts. Percentage of Caspase-3 (a), -7 (b) and Bax (c) expression in MDCK (B) and caspase-3 (a) and caspase-7 (b) HK2 (C) treated with LAAO-Bl for 12 h. Data normalized in relation to Control group. Protein bands were analyzed and normalized to the densitometric values of α-tubulin. Doxorubicin was used as positive control (10 μM). Data are representative results of three independent experiments.