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. 2015 Jul 20;10(7):e0133063. doi: 10.1371/journal.pone.0133063

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© 2015 Morgado et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) NETs containing neutrophil elastase (red) and amastigotes (green, arrow). (B) NETs containing histone (red) and amastigotes (green, arrow). (C) Area enclosing intact amastigotes (green—arrowhead) in intimate contact with NETs labeled with anti-neutrophil elastase (red). Area containing free Leishmania sp antigens (arrow). Below each image the respective side view (Z images) of (a1, b1, c1) amastigotes (green), (a2, c2) NET-elastase, (b2) NET-histones, merged sections showing the close contact between amastigotes and NETs (a3, b3, c3). Scale bar = 10μm.