Fig. 2. Hoxa10, Hoxa11 and Hoxa13 are the only members of the HoxA cluster expressed in the allantois.
Wild-type expression patterns of Hoxa genes as revealed by whole-mount in situ hybridization on E7.5 (top), E8.5 (middle) and E9.5 (bottom) mouse conceptuses. At E7.5 and E8.5, only the most 5′ genes (Hoxa10, Hoxa11 and Hoxa13) are expressed in the allantois (black arrows). Note that these genes are expressed prior to genes located at more 3′ positions. At E8.5, all Hoxa genes are transcriptionally activated, but none of the group 1 to 9 Hoxa genes is expressed in the allantois (white arrows). By contrast, 5′ Hoxa expression in the allantois is barely, if at all, detectable in E9.5 allantois and there is no evidence for Hoxa expression in the labyrinth (bottom panel, dashed ovals).