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. Author manuscript; available in PMC: 2015 Jul 20.
Published in final edited form as: Development. 2012 Jan 4;139(4):731–739. doi: 10.1242/dev.075408

Fig. 6. Expression of 5′ Hoxa genes in chick allantois and evidence for a shared allantois enhancer in mice.

Fig. 6

(A) Whole-mount in situ hybridization on chick embryos at stage HH18. Hoxa13 and Hoxa11 are expressed in the allantois (black arrows), whereas Hoxa9 is not (white arrow), illustrating that 5′ Hoxa expression in the allantois is not restricted to placental species. (B–E) Schematic representation of the wild-type HoxA cluster (B) and alleles carrying rearrangements or deletions within the HoxA cluster (C–E). For each allele, the expression pattern for 5′ Hoxa genes, Evx1 or PGK transgenes is shown. (B) Wild-type expression of Hoxa13. (C) The PGK promoter is activated in the allantois when inserted at the 5′ end of the HoxA cluster (HoxAflox allele). (D) The PGK transgene remains expressed in the allantois even in the absence of the entire HoxA cluster (HoxAdelneo+). (E) Evx1 expression remains excluded from the allantois even when the HoxA cluster is deleted (HoxAdel allele). Wild-type expression of Evx1 (black box). (F) The IR50 transgene, which contains the 50 kb Hoxa13-Evx1 intergenic region linked to the minimal promoter and lacZ reporter, is expressed in the allantois.

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