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. 2014 May 6;18(6):966–974. doi: 10.1111/jcmm.12293

Figure 1.

Figure 1

Production and characterization of miR-483. (A) Alignment of pre-miR-483 sequences from different species (primates and rodents). White characters indicate the pre-miR-483 sequence, black background indicates homology, and grey background indicates different nucleotides. (B) The construct for the miR-483 transgene. miR-483 was cloned between the two EcoR I sites, which puts the transgene under the control of the CAG promoter. The construct was then injected into the male pronuclei of the oocytes of pregnant C57BL/6 mice. (C) Reverse transcriptional PCR was used to detect the expression of the transgene in the liver. (D) The expression of the transgene was assessed by qRT-PCR on total RNA extracted from the livers of 2-month-old mice. **P < 0.01.