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. 2014 May 6;18(6):966–974. doi: 10.1111/jcmm.12293

Figure 4.

Figure 4

miR-483 down-regulates platelet-derived growth factor-β (PDGF-β) and tissue inhibitor of metalloproteinase 2 (TIMP2). (A) The sequence sites of miR-483 and the binding UTR of PDGF-β and TIMP2. (B) miR-483 inhibits the expression of PDGF-β after transfection in vitro. Immunohistochemistry (C) and Western blotting (D) for TIMP2 and PDGF-β in transgenic and wild-type mice liver. Overexpression of miR-483 decreased the translation of TIMP2 and PDGF-β. (E) The schematic diagram shows luciferase constructs containing each putative miR-483 binding site with or without mutation. (F and G) The luciferase activity assay with the mouse TIMP2 and PDGF-β UTRs. miR-483-3p and miR-483-5p reduced the expression of TIMP2 and PDGF-β by targeting their UTR; *P < 0.05, **P < 0.01.