Figure 6.

The percentage of MKN28 cells staying in each phase of the cell cycle in low Cl⁻ condition (A), low pH condition (B), bafilomycin A1 (BA; C) or ethyl isopropyl amiloride (EIPA; D). Open columns indicate the percentage of MKN28 cells cultured under normal condition for low Cl⁻ condition (A) and low pH condition (B) or DMSO alone for BA (C) and EIPA (D). The closed column indicates the percentage of MKN28 cells cultured under low Cl⁻ condition (A), low pH condition (B), BA (C) and EIPA (D). The population of cells in the G₀/G₁ phase was increased and that in the S phase was significantly decreased by exposure to low Cl⁻ condition (A), BA (C), or EIPA (D) compared with normal condition for low Cl⁻ condition or DMSO alone for BA or EIPA. Low pH (B) condition caused no significant change in cell cycle compared with normal condition; n = 6, *P < 0.05.