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. 2014 Apr 12;18(6):1124–1133. doi: 10.1111/jcmm.12257

Figure 8.

Figure 8

Expression of cleaved caspase3, cleaved caspase9, Akt (total), phospho-Akt (Ser473) and GAPDH. (A) Expression of cleaved caspase3, cleaved caspase9, Akt (total), phospho-Akt (Ser473) and GAPDH by Western blotting. (B) Low Cl− condition caused a significant increase in cleaved-caspase9 compared with normal condition in MKN28 cells without any effect on cleaved-caspase3. On the one hand, low pH condition caused a significantly decrease in cleaved-caspase9 compared with normal condition in MKN28 cells without any effect on cleaved-caspase3. Bafilomycin A1 (BA) or ethyl isopropyl amiloride caused significant increases in both cleaved-caspase3 and cleaved-caspase9 compared with DMSO alone in MKN28 cells. (C) Low Cl− condition caused a significant increase in the ratio of phospho-Akt/Akt (total) compared with normal condition in MKN28 cells. On the other hand, BA caused a significant decrease in the ratio of phospho-Akt/Akt (total) compared with DMSO alone in MKN28 cells; n = 4, *P < 0.05.