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. 2014 Mar 17;18(6):1134–1143. doi: 10.1111/jcmm.12260

Figure 6.

Figure 6

AAPH exposure replicates the effect of caffeine on the developing eye. Early chick embryos exposed to 10 μmol/egg AAPH for 6 days and immunostained with NF antibody. (A and B) Control (A) and AAPH-treated (B) chick embryos. (C–E) NF immunostaining demonstrates the presence of neurons in control (C) and AAPH-treated (right eye, D) and (left eye, E) lens. The staining shows AAPH-treatment repressed neuron development in the eyes. (C'–E''') Transverse section of the lens at the levels indicated by white line in A and B. Bright-field (C', D', E'), NF immunostaining (C'', D'', E'') and NF immunostaining + DAPI (C''', D''', E'''). (O and P) Bar charts showing AAPH significantly increased the incidence of eye malformation (O) and reduced the size (diameter) of the eyes (P). NF, neurofilament. Scale bars = 2 mm in A and B; 1 mm in C–E; 200 μm in F–N.