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. 2014 Apr 24;18(6):1144–1156. doi: 10.1111/jcmm.12274

Figure 5.

Figure 5

Telocytes (TCs) did not exert any effect on renal TEC proliferation or apoptosis following ATP depletion in vitro. Neither TCs nor renal fibroblasts stimulated the proliferation of NRK-52E cells in a physically separated co-culture system (A and B). NRK-52E cells were co-cultured with renal TCs or FIBs in a MillicellTM system for the time periods indicated on the x-axis, as described in the Materials and Methods section. Proliferation was evaluated via the CCK-8 assay and through counting the number of viable cells in the culture. (B) ATP depletion insult caused reduced cell viability and an increase in the cell death rate based on the CCK-8 assay and cleaved caspase-3 immunofluorescence staining. The expression of cleaved caspase-3 was evaluated through semi-quantitative assessment (C–E). CTR: control (renal cells cultured without TCs or FIBs).FIB (renal cells cultured with FIBs). TCs(renal cells cultured with TCs).