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. 2014 Mar 18;18(6):1157–1171. doi: 10.1111/jcmm.12261

Figure 1.

Figure 1

Expression of XBP1S in the course of chondrogenesis in vitro. (A and B) Expression of XBP1S and Collagen X was examined in the course of chondrogenesis of a micromass culture of ATDC5 cells (A) and C3H10T1/2 cells (B). Micromass cultures of ATDC5 cells or C3H10T1/2 cells were stimulated with BMP2 protein for various time-points, respectively, as indicated, and the mRNA levels of XBP1S and ColX and GAPDH (serving as an internal control) were examined by real-time PCR. Rel. level, relative level. (C and D) Differential expression of XBP1S protein was examined by western blotting assay. After incubation of micromass cultures of ATDC5 cells (C) or C3H10T1/2 cells (D) with 300 ng/ml BMP2 for the various times indicated, the cells were lysed, and the levels of XBP1S and tubulin (serving as an internal control) were detected by using immunoblotting assay.