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. 2014 Mar 20;18(6):1213–1225. doi: 10.1111/jcmm.12268

Figure 4.

Figure 4

Calcitonin gene-related peptide (CGRP) expression in mouse dorsal root ganglion neurons after 48 hrs of culture. (A) Representative fluorescent images of neuronal cultures treated with 100 pg/ml nerve growth factor (NGF; +NGF, Row 1), 100 pg/ml NGF and normal IgG (+NGF IgG, Row 2), 100 pg/ml NGF and anti-NGF antibody (+NGF Ab, Row 3), media conditioned by degenerating, painful intervertebral disc (IVDs; DD, Row 4), or degenerating IVD media with anti-NGF antibody (DD Ab, Row 5). PGP 9.5 (green) is a general neuronal marker and CGRP (red) is pain neurotransmitter. PGP 9.5 and CGRP are overlaid in merged images. White arrows indicate CGRP-immunoreactive neurons; scale bar: 200 μm. (B) Quantification of CGRP immunoreactivity for each group. n = 2 samples per group, tested in duplicate, with five fields counted per duplicate totalling 10 fields counted per condition, error bars; ±95% CI, one-way anova. *P < 0.05.