Skip to main content
. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Brain Behav Immun. 2015 May 27;48:301–312. doi: 10.1016/j.bbi.2015.04.020

Figure 7. TLR2/1 and TLR2/6 heterodimer activation in glia-depleted cortical neurons in culture induces expression of immune-related genes.

Figure 7

(A) Gene array analysis of FSL1- and Pam3CSK4-treated neurons resulted in 36 genes co-regulated by both treatments, 8 genes uniquely altered by Pam3CSK4, and 55 genes uniquely altered by FSL1 treatment. Cortical neurons derived from E18 rat embryos were treated with Ara-c to prevent growth of astrocytes in the culture. (B) Neuronal cultures exhibit high purity of neurons with negligible glial cells. These neurons express mRNA for Timp1, Nfkbia, Cxcl1, C1s, Lcn2, Ccl2, IL17rb, Tf, Tlr1, Tlr2, Tlr6 and Tlr10. (C) FSL1 and Pam3CSK4-treated cortical neurons exhibit up regulation of the above genes. * P<0.05.