Skip to main content
. 2014 Oct 22;9(2):1117–1126. doi: 10.1021/nn5039433

Figure 5.

Figure 5

Conductance analysis of DNA nanopores embedded in lipid bilayers mounted across a nanopipette orifice. (A) Single-channel current trace of a pore in the high-conductance state at +40 mV. (B) IV curves for single DNA nanopores in the high and the low-conductance state and a lipid bilayer without pores as reference. The data spread for the two pore conductances can be estimated from the peak width in the all-point histograms in panel D. (C, D) Single-channel current trace fluctuating from the (C) low- and (D) high-conductance state to a completely closed pore. (E) Voltage-stepped current trace of a low-conductance state switching to complete and permanent pore closure at +80 mV. (F) Conductance histogram derived from single-channel current recordings at +100 mV. (G) Cumulative all-point histogram of 21 current traces from +20 to +100 mV in 20 mV steps. (H) Probability of observing the open state as a function of voltage. The probabilities were derived from all-point histograms as described in Figure 4. Filled squares show data from the nanopipette-mounted membrane, while empty triangles are data from planar bilayer recordings.