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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Hepatol. 2015 Mar 28;63(2):429–436. doi: 10.1016/j.jhep.2015.03.023

Fig. 5. FoxM1 binds to the promoter of CD44 and activates its expression.

Fig. 5

(A) Human HCC cells line Huh7 was transfected with control or FoxM1-siRNA #1. Seventy-two hours after transfection, total RNAs were assayed for FoxM1 and CD44 by quantitative RT-PCR. (B, C) Huh7 cells and Hep3B cells were transfected with control plasmid (pcDNA3) or plasmid expressing FoxM1. Forty-eight hours after transfection, total RNAs were compared for FoxM1 and CD44 mRNAs by semi-quantitative PCR assays. Cyclophilin-mRNA was assayed as loading control. (D) Upper panel, Putative FoxM1-binding sites in the human CD44 promoter are shown. The schematic also includes the location of the PCR primers (arrows) used in the chromatin-IP assays. Chromatin-IP assays indicating FoxM1 binding to the two proximal sites are shown (D).