Table 1.

mtDNA changes during life. List of publications analyzing the changes in mtDNA point mutations in human aging with the age of the subjects analyzed in the studies

Age	Tissue	Technique	mtDNA region (nucleotides)	Changes during life	Ref
28 to 99	Brain, blood	denaturing gradient gel electrophoresis and Cloning	D-loop, tRNAs, COI	Increased with age	[135]
1 to 70	Muscle	PCR-restriction fragment length polymorphism	MELAS and NARF related-positions	No correlation with age	[136]
1–90	Muscle, kidney, heart	PCR	3243	Increased with age	[137]
0 to 101	Fibroblast	denaturing gradient gel electrophoresis	D-loop	Increased with age	[60]
1 to 90	Muscle	denaturing gradient gel electrophoresis	D-loop, tRNA	Increased with age	[138]
6 to 82	Muscle	PCR-restriction fragment length polymorphism	189 and 408	Increased with age	[139]
69 to 82	Muscle	denaturing gradient gel electrophoresis	COII and 5 tRNAs	Increased with age	[51]
≤31/≥53	Brain	Cloning and Sequencing	COI	Increased with age	[140]
0 to 92	Skin	single strand conformation polymorphism	D-loop and COII	No correlation with age	[141]
50 to 109	Heart	Sequencing	D-loop	Increased with age	[142]
19 to 95	T cells	Heteroduplex Reference strand mediated conformation analysis	D-loop	No correlation with age	[143]
10to 91	Brain	Cloning and Sequencing	10999–12168	Increased with age in frontal cortex. No correlation in substantia nigra	[144]
4 to 97	Buccal cell, muscle	PCR-restriction fragment length polymorphism, Sequencing, Southern Blot	189	Increased with age	[145]
31	Brain	Sequencing	Whole mitochondrial genome	No correlation with age	[146]
25 to 80	Brain	Sequencing	Whole mitochondrial genome	Increased with age	[54] [82]