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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2015 Apr 20;85:33–44. doi: 10.1016/j.freeradbiomed.2015.04.012

Figure 1. Inclusion of the SOD1 3’UTR enhances luciferase reporter gene activity.

Figure 1

(A) Cells were transfected with pGL3-Promoter Vector, pGL3-promoter-SOD1 3’UTR sense or pGL3-promoter-SOD1 3’UTR antisense vector. 48 h after transfection, relative luciferase activity was analyzed. (B) Schematic representations of the different constructs used for luciferase assay. The firefly luciferase reporter gene (green box), the different segments of SOD1 3’UTR sequences (orange box) and the PolyA region (black box) are shown. Various regions of the SOD1 3’UTR were inserted downstream of the luciferase gene at the XbaI site. (C) A2780 and (D) PANC1 cells were transfected with each construct and assayed for luciferase activity after 48 hours. Data represent mean ± SEM of three independent experiments.