Table I.
Independent variables (factors) | Levels | ||
---|---|---|---|
−1 | +1 | ||
Numeric factors | Concentration of β-cyclodextrin (A) | 0.5 mg/ml | 4 mg/ml |
pH of β-cyclodextrin (B) | 3 | 8 | |
Concentration of alginate (C) | 0.1 mg/ml | 1 mg/ml | |
concentration of TMC (D) | 0.5 mg/ml | 2 mg/ml | |
pH of TMC solution (E) | 3 | 6 | |
Concentration of CaCl2 (F) | 5 mg/ml | 30 mg/ml | |
Dependent variables (responses) | Constrains | ||
Y 1 = size (nm) | Minimize | ||
Y 2 = PdI | Minimize | ||
Y 3 = Entrapment efficiency (EE%) | Maximize |