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Bdp1 region II cross-links to Brf1 and C128. (A) Bdp1-Brf1 photo-cross-linking. Bdp1 and the photo-cross-linked bands were detected by anti-Myc antibody (lanes 1 to 4). Anti-Flag antibody against tagged Brf1 confirmed its presence in the cross-linked polypeptide (lanes 5 to 8). (B) BPA-substituted residues Glu291, Asn292, and Lys295 in Bdp1 cross-linked with C128 (right), as confirmed by probing with anti-Flag antibody against Flag-tagged C128 in the fusion protein band (left).
