FIG 6.

Components of the TOP2β/PARP-1 complex are recruited to chromatinized MMTV and endogenous GR/BRG1-responsive promoters in a hormone-dependent manner. DNAPK, TOP2β, and PARP-1 are recruited to stably integrated MMTV and endogenous promoters in a GR/BRG1-dependent manner, as demonstrated by chromatin immunoprecipitation analysis (ChIP). Purified immunoprecipitated DNA fragments from treated SW-13/MMTV cells expressing GR and BRG1 were analyzed by real-time PCR using primer sets covering the nucleosome B promoter region of MMTV (A) and to the 3′ coding region of the integrated luciferase reporter (B) or gene-specific primers targeting the GRs of HSD11B2 (C) and PLZF (D). Normal IgG was used as a control for nonspecific interactions. Quantitative analysis was performed and results displayed as percentages of ChIP input. Values are shown as mean ± standard deviation from biological replicates.