FIG 8.

Ku and SWI/SNF subunits, BRG1 and BAF250a, cooccupy chromatin MMTV after stimulation with hormone. ChIP-reChIP analysis of integrated MMTV promoter in treated SW-13 cells expressing GR and BRG1 was performed. Cells were treated with hormone, formaldehyde cross-linked, and subjected to ChIP analysis. (A) For the primary ChIP, DNA immunocomplexes were isolated using antibodies specific for GR, BRG1, Ku70, or BAF250a. (B) ReChIP analysis was performed using Dex-treated samples from primary ChIP immunoprecipitated with GR, BRG1, Ku70, or BAF250a antibodies. Immunopurified DNA complexes from both ChIPs were reverse cross-linked and analyzed by real-time PCR using MMTV nucleosome B-specific primers. Quantitative analysis of data from primary ChIP (A) and from reChIP (B) was performed, with results displayed as percentages of ChIP input. The data are from a representative experiment with standard deviations of technical replicates.