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. 2015 Jul 20;35(16):2740–2751. doi: 10.1128/MCB.00285-15

FIG 4.

FIG 4

Sestrin2 upregulation contributed to AMPK activation. (A) Cells were treated with rotenone and immunoblotted for phosphorylated AMPK (Thr172) and total AMPK. (B) After sestrin2 knockdown, cells were treated with 20 nM rotenone and immunoblotted for phosphorylated AMPK (Thr172) and total AMPK (T-AMPK). (C) After sestrin2 overexpression, cells were immunoblotted for phosphorylated AMPK (Thr172). Band density of phosphorylated levels of AMPK (P-AMPK) relative to that of actin was analyzed by densitometry. Values are the means ± SEM from three independent experiments. *, P < 0.05; **, P < 0.01.