Table 1.
Strains, plasmids and primers used in this study
| Name | Description | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F/endA1 hsdR17 (rk- mk+) gin V44 thi-1 RecA1 gyrA (Nal1) relA1Δ (lacZYA-arg U169 deoR5 (ψ80dlacΔ (lacZ) M15)] | (Gibco BRL) |
| E. coli Ba | E. coli DH5α harboring pBatdds | (24) |
| E. coli Br | E. coli DH5α harboring pBatdds | (24) |
| E. coli BaTi | E. coli Br harboring pTispA | (This study) |
| E. coli BrTi | E. coli Br harboring pTispA | (This study) |
| E. coli BaDi | E. coli Ba harboring pDispA | (This study) |
| E. coli BrDi | E. coli Br harboring pDispA | (This study) |
| Plasmids | ||
| pTrc99A | Ptrc expression vector, pBR322 origin, lac/q, Ampr, high copy | (Amersham, Bioscience) |
| pBBR1MCS2 | Plac cloning vector, pBBR1 origin, lacZα, Kanr | (37) |
| pBAD24 | PBAD cloning vector, pBR322/ColE1 origin, araC, Ampr | (38) |
| pBatdds | pBBR1MCS2 with dds gene from A. tumefaciens | (24) |
| pBrsdds | pBBR1MCS2 with dds gene from R. sphaeroides | (24) |
| pTispA | pTrc99A with ispA gene from E. coli | (This study) |
| pDispA | pBAD24 with ispA gene from E. coli | (This study) |
| Primers | ||
| Atdds-3 | 5′-TCCCCCCGGGCACAGGAAACAGACCATGGAGTTCTTG-3′ (XmaI) | |
| Atdds-4 | 5′-TCCCCCCGGGTTAGTTGAGACGCTCGATGCAGAAG-3′ (XmaI) | |
| Rsdds-F | 5′-TCCCCCCGGGTTAGTTGAGACGCTCGATGCAGAAG-3′ (XmaI) | |
| Rsdds-R | 5′-TAAGAGCTCAAGGGATCAGGCGATGCGTTCGAC-3′ (SacI) | |
| ispA-F | 5′- ATAGAATTCAGGAGGAATGAGTAATGGAC-3′ (EcoRI) | |
| ispA-R | 5′-ATAGGATCCGGTACCTCATTATTTATTACGC-3′ (BamHI) |